Which method for ethanol measurement is described as enzymatic?

Which method for ethanol measurement is described as enzymatic?

• A. Enzymatic method
• B. Gas chromatography
• C. Immunoassay
• D. Colorimetric assay

Answer: (A)

Enzymatic measurements of ethanol rely on a specific enzyme, typically alcohol dehydrogenase, to catalyze the oxidation of ethanol to acetaldehyde with NAD+ being reduced to NADH. The amount of NADH produced is proportional to the ethanol concentration and is detected spectrophotometrically by measuring absorbance at 340 nm, or via a linked colorimetric readout that tracks NADH formation. This enzyme-driven reaction is what defines an enzymatic method for ethanol measurement. Gas chromatography separates components and quantifies ethanol without using a catalytic enzyme. Immunoassays detect ethanol or its metabolites through antibody binding rather than enzyme activity. Colorimetric assays describe a readout based on a color change and may or may not involve enzymes, but the defining enzymatic approach is the one that uses an enzyme-catalyzed reaction to generate the measurable signal.

Enzymatic measurements of ethanol rely on a specific enzyme, typically alcohol dehydrogenase, to catalyze the oxidation of ethanol to acetaldehyde with NAD+ being reduced to NADH. The amount of NADH produced is proportional to the ethanol concentration and is detected spectrophotometrically by measuring absorbance at 340 nm, or via a linked colorimetric readout that tracks NADH formation. This enzyme-driven reaction is what defines an enzymatic method for ethanol measurement. Gas chromatography separates components and quantifies ethanol without using a catalytic enzyme. Immunoassays detect ethanol or its metabolites through antibody binding rather than enzyme activity. Colorimetric assays describe a readout based on a color change and may or may not involve enzymes, but the defining enzymatic approach is the one that uses an enzyme-catalyzed reaction to generate the measurable signal.